The investigation of lipoprotein-related phospholipase A2 (LP-PLA2) V279F mutation in coronary artery disease

Authors

  • Hatice Yildirim Yaroglu Mersin University, Institute of Health Science, Department of Stem Cell and Regenerative Medicine, Mersin, Türkiye
  • Nazli Akciger Mersin University, Faculty of Medicine, Department of Cardiology, Mersin, Türkiye
  • Mehmet Ali Sungur Düzce University, Faculty of Medicine, Department of Biostatistics and Medical Informatics, Düzce, Türkiye
  • Dilek Cicek Yilmaz Mersin University, Faculty of Medicine, Department of Cardiology, Mersin, Türkiye
  • Lulufer Tamer Mersin University, Faculty of Medicine, Department of Biochemistry, Mersin, Türkiye

Keywords:

Lp-PLA2, Coronary artery disease, V279F

Abstract

Aim: Despite advances in the treatment of cardiovascular diseases, coronary artery disease (CAD) is the leading cause of death worldwide. The media layer of normal and diseased arteries contains lipoprotein-associated phospholipase A2 (Lp-PLA2), which is mostly made by mast cells, T lymphocytes, and monocytes. Our research aimed to define how the Lp-PLA2 and V279F gene polymorphisms are related to CAD.

Materials and Methods: This study included 109 CAD patients and 71 controls. Fasting blood glucose (FBG) and lipid profiles were measured via the enzymatic colorimetric technique. Lp-PLA2 levels were detected with ELISA. The genomic DNAs were isolated from whole blood. Real-time polymerase chain reaction (RT-PCR) with the V279F mutation detection kit was used to identify the Lp-PLA2 V279F mutation. 

Results: In the CAD group compared to controls, serum Lp-PLA2 levels were higher (p 0.001). While FF (mutant) genotypes and VF (heterozygous) genotypes were absent in both groups, the Lp-PLA2 V279F mutation was discovered as the VV (wild) genotype in both the CAD group and controls.  

Conclusion: As a result, we determined that the Lp-PLA2 V279F mutation cannot be considered a genetic risk factor for CAD, but that Lp-PLA2 elevation may be an important parameter for CAD.

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Published

2023-10-25

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Section

Original Articles

How to Cite

1.
The investigation of lipoprotein-related phospholipase A2 (LP-PLA2) V279F mutation in coronary artery disease. Ann Med Res [Internet]. 2023 Oct. 25 [cited 2025 Apr. 2];30(10):1217-22. Available from: http://annalsmedres.org/index.php/aomr/article/view/4561