Investigation of the toxicity of acetamiprid in SH-SY5Y neural cells

Authors

  • Ilker Deniz Cingoz Usak University, Faculty of Medicine, Department of Neurosurgery, Usak, Turkey
  • Ismail Kaya Usak University, Faculty of Medicine, Department of Neurosurgery, Usak, Turkey
  • Hulya Birinci a3Gaziantep University, Faculty of Medicine, Department of Histology and Embryology, Gaziantep, Turkey
  • Suna Karadeniz Saygili Kutahya Health Sciences University, Faculty of Medicine, Department of Histology and Embryology, Kutahya, Turkey
  • Mustafa Oztatlici Celal Bayar University, Faculty of Medicine, Department of Histology and Embryology, Manisa, Turkey

Keywords:

Acetamiprid, apoptosis, human neuroblastoma cells, oxidative stress, neurotoxicity

Abstract

Aim: Acetamiprid (ACE) is one of the most widely used neonicotinoids globally to protect crops from insects. In this study aimed to investigate the potential neurotoxic activity of acetamiprid on human neuroblastoma cell line SH-SY5Y cells.

Materials and Methods: MTT and Muse analysis were performed to examine the effect on cell viability. Increasing doses of ACE were administered for 24 hours in SHSY5 neuroblastoma cell lines. NOS1, NOS2, NOS3; caspase-3 for assessment of apoptosis; Ki67 immunocytochemical staining was performed to evaluate proliferation, and relative mRNA values of these markers were measured by qRT-PCR analysis method to evaluate the efficacy of ACE on oxidative stress in neuroblastoma cell lines

Results: The IC50 value for ACE 24 hours was found to be 21.35 mM. In SHSY5 cells, the immunoreactivity of NOS1, NOS3, and caspase-3 markers in the ACE applied group increased statistically significantly compared to the control group; Ki67 immunoreactivity also decreased (p< 0.05). qRT-PCR results were consistent with immunocytochemical findings, and relative mRNA values increased in ACE groups compared to the control group. Ki67 relative mRNA values decreased compared to the control group.

Conclusion: In our study, it was found that ACE suppressed proliferation in SH-SY5Y cells, induced apoptosis, and caused cell toxicity by increasing oxidative stress.

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Published

2022-05-26

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Section

Original Articles

How to Cite

1.
Investigation of the toxicity of acetamiprid in SH-SY5Y neural cells. Ann Med Res [Internet]. 2022 May 26 [cited 2025 Apr. 2];29(5):509-14. Available from: http://annalsmedres.org/index.php/aomr/article/view/4133