The effect of ouabain on histamine release from human skin mast cells

Authors

  • Mustafa Senol
  • I. Halil Ozerol
  • Asha V. Patel
  • David P. Skoner

Keywords:

Calcium, calcium ionophore, histamine, Na -K ATPase, ouabain

Abstract

There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+ -K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+ -K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies utilized animal-derived cells. The aim of this study was to determine the effect of Na+ -K+ ATPase inhibition on human skin mast cells. Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 µM) for 30 min following a 1 hr incubation with various concentrations (10e-4 to 10e-8 M) of ouabain. Histamine release was assayed by enzyme immunoassay. The results indicated that ouabain, a Na+ -K+ ATPase inhibitor, had no significant effect on the non-immunologic histamine release from human skin mast cells in vitro and suggested differences between human and animal mast cells.

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Published

2021-05-25

Issue

Section

Original Articles

How to Cite

1.
The effect of ouabain on histamine release from human skin mast cells . Ann Med Res [Internet]. 2021 May 25 [cited 2025 Feb. 24];3(4):0277-83. Available from: http://annalsmedres.org/index.php/aomr/article/view/3690