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Formerly Journal of Turgut Ozal Medical Center

  • Annals of Medical Research



    Annals of Medical Research International Monthly Published Medical Journal

  • Annals of Medical Research

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  • Annals of Medical Research


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  • Annals of Medical Research
  • Annals of Medical Research
  • Annals of Medical Research
  • Annals of Medical Research

Dear Annals of Medical Research Readers

As of the end of March 2021, I have started to work as Chief Editor at Annals of Medical Research. Our journal is the official publication of Inonu University Medical Faculty and published uninterruptedly for 28 years.  Our Journal, which is online as an open access journal with monthly free access, is being scanned in many national and international indexes (ULAKBIM TR Dizin, Turkish Medline, EBSCO, Google Scholar, etc.). I would like to thank the Editors before me who contributed to this level. Our primary goal is to ensure the maintenance of this good state of the journal and to ensure that it is scanned in prestigious international indexes (Pubmed Central, SCI-Expended, etc.). In order to achieve these goals, it depends on the original research articles that can be cited and the articles published in our Journal to be cited sufficiently in other international journals. The contributions of our readers, reviewers and authors in achieving this goal are of great importance.

I hope to announce the news that we have reached our goals as soon as possible.

MD. Prof. Nevzat Erdil

Editor-in-Chief

Annals of Medical Research

 

Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTX and IntI in Klebsiella pneumoniae (p.197-203)

Analyses of the relationships among biofilm activity, antibiotic resistance and expressions of SHV, TEM, CTX and IntI in Klebsiella pneumoniae (p.197-203)

Ridvan Guckan1, Cetin Kilinc1, Ceren Baskan2, Burak Yazgan3, Seda Mesci4, Tuba Yildirim5
1Microbiology Laboratory, Amasya Sabuncuoglu Serefeddin Education and Research Hospital, Amasya, Turkey
2Department of Physical Therapy and Rehabilitation, Sabuncuoglu Serefeddin Health Services Vocational School, Amasya University, Amasya, Turkey
3Department of Medical Services and Techniques, Sabuncuoğlu Serefeddin Health Services Vocational School, Amasya University, Amasya, Turkey
4Department of Medical Biochemistry, Faculty of Medicine, Hitit University, Corum, Turkey
5Department of Biology, Faculty of Arts and Sciences, Amasya University, Amasya, Turkey
Copyright@Author(s) - Available online at www.annalsmedres.org
Content of this journal is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Abstract

Aim: Klebsiella pneumoniae as an opportunistic pathogen is responsible for nosocomial, urinary tract infections, pneumonia, bacteremia, liver abscesses, and respiratory and blood infections in patients.
The aim of our study is to examine the antibiotic sensitivity, expression levels of IntI, TEM, SHV and CTX genes, which play a role in multidrug resistance, biofilm formation and even the relationships among them.
Materials and Methods: Identification was implemented by carrying out the VITEK2 system and phenotypic confirmation of the ESBL producing isolates by using a combined disc test. Antibiotic sensitivity was implemented by disc diffusion method and microdilution methods toward various antibiotics. Furthermore, biofilm formation was examined through microtitration plate method.
Results: The expressions of IntI, TEM, SHV and CTX were implemented in qRT-PCR. 19 were susceptible to all antibiotics, 17 were ESBL (+) and 16 were carbapenem-resistant among 52. The expression of these genes was upregulated in ESBL (+) strains and produced biofilm and the expression of these genes was upregulated, too.
Conclusion: The results of the study revealed the potential role of the mentioned genes in biofilm formation and antibiotic resistance, enabling the development of new drugs. Further studies will be essential so as to determine when or how these systems are included in antibiotic resistance and biofilm formation.

Keywords: Klebsiella pneumoniae; biofilm; IntI; TEM; SHV; CTX

 

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doi:10.5455/annalsmedres.2020.03.211

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Annals of Medical Research is the scientific official journal of the Inonu University Faculty of Medicine. It is published monthly. The essential aim of the Annals of Medical Research is to publish scientifically high quality clinical and experimental research articles on fields of medicine which can contribute to the literature data. In addition, review articles, case reports, editorials, letters to the editors and manuscripts on publication ethics and medical history, which can contribute to the education and practices of physicians and health sector professionals within the scope of the journal’s target audience and which can increase the level of scientific communication between the authors and readers, are included in the scope of the journal.

 

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