1Abant Izzet Baysal University, Faculty of Medicine, Department of Orthopaedic and Traumotology, Bolu, Turkey
2Namik Kemal University, Faculty of Medicine, Department of Neurosurgery, Tekirdag, Turkey
3Istanbul Medipol University, Faculty of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
4Namik Kemal University, Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, Tekirdag, Turkey
5Memorial Bahcelievler Spine Center, Clinic of Neurosurgery, Istanbul, Turkey
6Istanbul Medipol University School of Medicine, Department of Medical Pharmacology, Istanbul, Turkey
7Istanbul Koc University Hospital, Clinic of Neurosurgery, Istanbul, Turkey
Aim: Although antibiotics are generally well-tolerated, they may have cytotoxic effects. The present randomized, double-blind, in vitro study aimed to investigate the effects of tigecycline on cartilage tissue cells and the extracellular matrix.
Material and Methods: Cartilage tissues of patients (n = 8) were used for the preparation of primary cell cultures. Tigecycline-treated cell cultures served as the study group. Non-treated cell cultures served as the control group. Analyses were performed at 0, 24, 48, and 72 h in both groups. The results obtained were statistically evaluated. The alpha significance value was determined to be 0.05.
Results: Proliferation remained unchanged in the tigecycline-treated cell cultures. The gene expressions of the markers involved in anabolic pathways increased in the tigecycline-treated cell cultures. The results obtained were statistically significant (P 0.05).
Conclusion: Although tigecycline had no toxic effect on the chondrocyte cell cultures and caused no damage to the extracellular matrix, the present study was performed in an in vitro environment.
Keywords: Chondroadherin gene; Cartilage oligomeric protein; Interleukin-1beta; Matrix metalloproteinase; Primary chondrocyte culture; Tigecycline