The effect of ouabain on histamine release from human skin mast cells

Main Article Content

Mustafa Senol
I. Halil Ozerol
Asha V. Patel
David P. Skoner

Abstract

There are controversial reports on the effect of sodium-potassium adenosine triphosphatase (Na+ -K+ ATPase) inhibition on mast cell mediator release. Some of them have indicated that ouabain (strophanthin G), a specific Na+ -K+ ATPase inhibitor, inhibited the release, whereas the others have shown that ouabain had no effect or even had a stimulatory effect on the mediator secretion. Most of these studies utilized animal-derived cells. The aim of this study was to determine the effect of Na+ -K+ ATPase inhibition on human skin mast cells. Unpurified and purified mast cells were obtained from newborn foreskins and stimulated by calcium ionophore A23187 (1 µM) for 30 min following a 1 hr incubation with various concentrations (10e-4 to 10e-8 M) of ouabain. Histamine release was assayed by enzyme immunoassay. The results indicated that ouabain, a Na+ -K+ ATPase inhibitor, had no significant effect on the non-immunologic histamine release from human skin mast cells in vitro and suggested differences between human and animal mast cells.

Downloads

Download data is not yet available.

Article Details

How to Cite
Senol, M., Halil Ozerol, I., V. Patel, A., & P. Skoner, D. (2021). The effect of ouabain on histamine release from human skin mast cells . Annals of Medical Research, 3(4), 0277–0283. Retrieved from https://annalsmedres.org/index.php/aomr/article/view/3690
Section
Original Articles

Most read articles by the same author(s)

1 2 3 > >>