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Formerly Journal of Turgut Ozal Medical Center - ISSN 1300-1744

eNOS gene polymorphısms and transıtıonal cell cancer of the bladder (p.2004-10)

eNOS gene polymorphısms and transıtıonal cell cancer of the bladder (p.2004-10)

Aziz Toker1, Erkan Erkan1, Fatma Sinem Hocaoglu Emre2, Ugur Yucetas1
1Clinic of Urology, Istanbul Education and Reseach Hospital, Istanbul, Turkey
2Department of Nutrition and Dietetics, Beykent University, Istanbul, Turkey
Copyright © 2020 by authors and Annals of Medical Research Publishing Inc.

Abstract

Aim: In this case-control study, we examined the association between two types of eNOS gene polymorphisms (intron 4 a/b and Glu298Asp) and transitional cell carcinoma (TCC) of the bladder.
Nitric Oxide (NO) is a free radical that plays a key role in various physiological and pathophysiological processes, particularly in the circulatory system. Endothelial Nitric Oxide Synthase (eNOS), as a member of NOS family, is the enzyme, responsible for the physiological production of nitric oxide in the endothelium. Polymorphisms of eNOS have been related to increased risk for different types of cancers.
Material and Methods: A total of 64 patients with bladder TCC and 80 controls with similar epidemiological characteristics of patients group were evaluated and compared in terms of eNOS gene polymorphisms of two different types ((intron 4 a/b and Glu298Asp). Following DNA isolation from the blood samples, eNOS gene was replicated via PCR. The genomic distribution of two types of eNOS polymorphism was determined by gel electrophoresis following the process of restriction, for both groups.
Results: The alleles most commonly observed in patient group were “ab” for intron 4 (OR= 1.80, 95% CI: 0.71-4.64; P=0.20) and “GT” for Glu298Asp polymorphisms (OR= 1.65, 95% CI: 0.85-3.22; P=0.14). Neither bladder cancer risk nor disease grade was associated with these polymorphisms.
Conclusions: This study suggests that the intron 4 a/b and Glu298Asp eNOS gene polymorphisms are not associated with bladder cancer susceptibility. However, it is mandatory to conduct further trials with more patients to confirm these findings and to evaluate the association between bladder cancer and eNOS gene polymorphisms.

Keywords: Bladder cancer; Glu298Asp polymorphism; intron 4 a/b  polymorphism; Nitric oxide synthase; Nitric oxide synthase polymorphism

 

 

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doi: 10.5455/annalsmedres.2020.01.039       

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Annals of Medical Research is the scientific official journal of the Inonu University Faculty of Medicine. It is published monthly. The essential aim of the Annals of Medical Research is to publish scientifically high quality clinical and experimental research articles on fields of medicine which can contribute to the literature data. In addition, review articles, case reports, editorials, letters to the editors and manuscripts on publication ethics and medical history, which can contribute to the education and practices of physicians and health sector professionals within the scope of the journal’s target audience and which can increase the level of scientific communication between the authors and readers, are included in the scope of the journal.

 

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