The effect of oral puerarin administration on wound healing in diabetic rat model
Akif Turer1, Mehmet Emin Onger2
1Bulent Ecevit University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Zonguldak, Turkey
2Ondokuz Mayıs University, Faculty of Medicine Department of Histology and Embryology, Samsun, Turkey
Aim: The aim of this study is to examined the effect of the oral administration of puerarin on wound healing in diabetic rat.
Material and methods: Twenty-four rats were divided into three groups: Group 1 (n:8) non diabetic control (NDC), group 2 (n:8) diabetic control (DC), group 3 (n:8) diabetic puerarine (DP). Rats Group DC and DP were injected a single dose 200 mg/kg streptozin solution for diabet model. A full thickness linear incision was made in the paravertebral region of the rats and the wound edges were covered with a sterile plaster. In Group NDC and DC, rats were given saline solution for 7 days. In Group DP, rats were given 200 mg/kg puerarin with oral gavage for 7 days. All animals were euthanized at 7 days postoperative. Connective tissue volumes and new vessel formation were measured with stereological analysis
Results: Stereologic analysis showed that Group NDC and DP had significantly more connective tissue volume at 7 days compared with group DC. New vessel formation volumes were also significantly lesser in group DC. Connective tissue and new vessel volumes difference were not statistically significant between group NDC and DP.
Conclusion: Orally administered puerarin enhances new vessel formation and support wound healing in diabetic rats.